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Metabolic profiling and metabolomics

Our research led us to perform detailed analyzes of metabolic processes associated with the allocation of carbon and nitrogen assimilates in the plant and adjustments implemented in response to abiotic stresses. Investigations are directed towards targeted analyzes of nitrogen and carbon metabolites derived from primary metabolism or towards non-targeted metabolic profiling and fingerprinting approaches. These steps are performed on the analytical platform P2M2.

1) Targeted analysis of primary C and N metabolites

Measuring the efficiency of nitrogen utilization and processes of carbon and nitrogen resource recycling in the vegetative parts and reproductive organs, both under optimal and abiotic stress conditions, lead us to pay attention to the qualitative and quantitative levels of free and proteolytic aminoacids, non structural sugars and organic acids, amines and polyamines, phytohormones, fatty acids,…

Aminoacid profile of Arabidopsis leaf tissues through UHPLC-DAD

Aminoacid profile of Arabidopsis leaf tissues through UHPLC-DAD

2) Non-targeted metabolic profiling and fingerprinting analyses

Comparative metabolite of Arabidopsis thaliana and Thellungiella salsuginea (halophila) through GC-MS

The search for new determinants of nutritional, water, salt, temperature stress tolerance, leads us, both in oilseed rape and Arabidopis relative model species (Arabidopsis sp., Thellungiella sp., Arabis sp.) to develop comprehensive metabolomic approaches through appropriate NMR spectroscopy or mass spectrometry procedures.

Arabidopsis thaliana and Thellungiella salsuginea (halophila) under increasing salt treatments

Comparative metabolite of Arabidopsis thaliana and Thellungiella salsuginea (halophila) through GC-MS under increasing salt treatments  (from Lugan et al., 2010)

References

Lugan R., Niogret M.F., Kervazo L., Larher F.L., Kopka J., Bouchereau A., 2009 - Metabolome and water status phenotyping of Arabidopsis under abiotic stress cues reveals new insight into ESK1 function. Plant Cell Env., 32, 95–108.

Lugan R., Niogret MF., Leport L.,  Guégan JP.,  Larher F., Savouré A.,  Kopka J.,  Bouchereau, A., 2010 - Metabolome and water homeostasis analysis of Thellungiella salsuginea suggests that dehydration tolerance is a key response to osmotic stress in this halophyte. Plant J. 64, 215-229. DOI

Renault H., El Amrani A., Berger A., Mouille G., Soubigou-Taconnat L., Bouchereau A., Deleu C., 2013 - γ-Aminobutyric acid transaminase deficiency impairs central carbon metabolism and leads to cell wall defects during salt stress in Arabidopsis roots. Plant Cell Environ. DOI