Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal Agrocampus Ouest Rennes 1 University

Home page

Are saliva proteins key determinants of host plant specificity in the pea aphid complex?

APHISPIT

  • Marie Curie Carrier Integration Grants, 4 years project, started 2013
  • Ph.D. project of Hélène Boulain funded by INRA SPE and Région Bretagne
  • Principal researcher: Akiko Sugio
  • Contacts: akiko.sugio@inra.fr

Research

Context

Aphids are insect pests that feed on plant phloem sap and cause feeding damages and transmission of plant pathogens. The pea aphid (Acyrthosiphon pisum) complex is consisted of multiple biotypes each of which specialized to one or a few specific legume plants. Aphids use a specialized mouth part to puncture plant phloem sieve cells and suck plant nutrients. During the feeding process, aphids secrete saliva which contains various proteins that might interact with plant proteins.

Objectives

This project aims to examine the roles of aphid salivary proteins in host plant adaptation. Based on previous works in plant microbe interactions, I hypothesize that aphid saliva proteins function like microbial pathogen effectors. Thus, I anticipate that the saliva proteins that are involved in host adaptation process are under high evolutionary pressure and show polymorphisms between biotypes.

Methodology

We will examine pool-seq data of aphid genomes (3 biotypes, 60 individual aphids/pool) to identify fast evolving salivary genes. Furthermore, we will conduct in-depth analysis of published and unpublished salivary proteomes and transcriptomes to extend the catalogue of the pea aphid salivary genes. The fast evolving salivary genes will be expressed in host/non host plants or silenced in aphids to examine their involvement in specific plant-aphid interactions.

Perspectives

The in-depth study of the pea aphid salivary genes will be extended to understand the evolutionary history of salivary genes in each aphid species. Functional analyses of salivary proteins will increase our knowledge on plant-aphid interactions at a molecular level and contribute to create/construct aphid resistant/tolerant crops.

Funding and Support

  • European Commission (100k€)
  • INRA SPE and Région Bretagne