Fish regeneration after germ cell grafting

Fish regeneration after germ cell grafting

Our research aims to improve the yield of germinal stem cell grafting, by characterizing the cellular factors favorable to the germ cell incorporation into the recipient gonad and to the proliferation and differentiation of the germinal stem cells in the recipient gonad, and by optimizing the universal sterile recipient which will sustain the development of gamete from the donor fish only.

Germinal stem cells in the adult males have a major interest because higher numbers of them can be collected in the adult testis than in the embryo or fry. Moreover, these cells are bipotent, meaning that they can differentiate into spermatozoa when grafted into a male fry, or in ova when grafted into a female one. Regenerating fish from these cells is a unique way to restore the whole genome of the donor fish, including the mitochondrial genome that is transmitted by the ova only. Cryopreservation of these cells is a way to bypass the inability of the fish ova or embryo to be cryopreserved.

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Modification date : 13 February 2023 | Publication date : 04 December 2017 | Redactor : Catherine Labbé