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INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

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Internet de l'unité STLO Science et Technologie du Lait et de l'Oeuf

STLO Research Unit

Multiscale analysis of protein changes induced by ultrafiltration

Multiscale analysis of protein
A package of complementary analyses examines the putative effects of ultrafiltration on protein structure and functionality.

Up to now, the impact of ultrafiltration on protein properties was mostly investigated via a unique technique. This is a great drawback for an accurate estimation of the filtration effect on protein properties due to the distinct sensitivity threshold from one analysis to the other.

We propose an original and complete analytical package. Structural and functional analyses are combined to examine protein changes at both macroscopic and molecular levels. This package is shown as pertinent for both dead-end and crossflow modes of filtration. Results correspond with data obtained from classical techniques.

Representation of the analytical package proposed Green terms stand for adaptations or new methods

Representation of the analytical package proposed.
(Green terms stand for adaptations or new method)

This original approach is relevant for investigating filtration mechanisms and understanding the modifications induced by ultrafiltration on globular milk proteins, even in mixed solutions.

 

FiletGris

 

Collaboration

This research was conducted with the financial support of the French National Research Agency (ANR), project ECOPROM ANR-06-PNRA-015.

Read more

Van Audenhaege, M., Belmejdoub, J., Dupont, D., Chalvin, A., Pezennec, S., Le Gouar, Y., Garnier-Lambrouin, F., Rabiller-Baudry, M., Gésan-Guiziou, G. (2010) A methodology for monitoring globular milk protein changes induced by ultrafiltration: a dual structural and functional approach. J. Dairy Sci., 93:3910-3924.
http://dx.doi.org/10.3168/jds.2009-2995

Contact

Geneviève Gesan-Guiziou • Genevieve.Gesan-Guiziou@rennes.inra.fr

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